Sterilization time of autoclave

High pressure steam sterilizer is a kind of equipment that uses saturated steam to quickly and reliably sterilize or sterilize articles. It is widely used in medical and health systems, biotechnology, food and drug production and processing. Sterilization and sterilization of medical devices, dressings, glassware, and solution culture media also apply to high-altitude drinking water in highland areas for cooking equipment and enterprises and institutions, as well as high-temperature steam source equipment.

 

Sterilization time of autoclave:

 

  1. Heat penetration time is the time required to reach the sterilization temperature from the sterilization temperature in the disinfecting cabinet to the center of the disinfected article. The length of time depends on the nature of the disinfectant, the size of the package, the location and the degree of air emptying in the autoclave and the type of sterilizer.

2, disinfection and maintenance time, that is, the time required to kill microorganisms, generally used to kill spores of T. stearothermophilus spores required time to express. It takes 30 minutes at 115°C, 12 minutes at 121°C, and 2 minutes at 132°C.

3, safety time, generally half of the maintenance time, depending on the length of the disinfection items. Sterilization of easily thermally conductive metal devices requires no safety time.

 

Daily maintenance and maintenance of pressure steam sterilizer


1. Wipe the surface of the sterilizer door and instrument daily, and clean the floor between the sterilization chamber at least once.
2. Clean the impurities in the strainer at the drain port of the sterilizer daily to prevent impurities from entering the exhaust pipe during sterilizer operation.
3. Check whether the door seal of the sterilizer is smooth, intact, and not removed or damaged before daily operation.
4. Check the accuracy of the meter pointer every day, observe whether the temperature meter and pressure instrument pointer is in “0” position after the sterilizer operation is stopped; observe whether the steam, water and other medium pipelines and valve parts have leaked; Bacteria operation indicator is in good condition; once it is found that there is a problem with the above components, the sterilizer should not be used. It should be used after maintenance.
5. Clean the wipe inside the sterilizer weekly and clean thoroughly.
6. Clean the exterior of the sterilization equipment quarterly to avoid dust accumulation and shorten the life of the air filter. Components should be kept out of contact with water. Once the wet water has dried, turn on the power supply.
7. Every quarter, check whether the outlets and connectors of each connection are loose and loose.
8. Clean the safety valve surface every 6 months.
9. Check the steam line filter once a week and record the results.

10. Each year, the pressure steam sterilizer is tested once a year, and safety valves, pressure gauges, and thermometers are validated at least once a year. The inspection results are recorded and retained. The air filter should be replaced regularly.

High Temperature Pressure Steam Medical Autoclave

How to maintain the laboratory bench

We often care about the quality of Laboratory Furniture, which is a normal requirement. But you ignore the problems on the other hand, such as staff mesa material selection for laboratory work station, if choose the corrosion-resistant chemical planks, will pay attention to use items, otherwise it is a good material can also reduce the service life; Include lightweight corrosion-resistant chemical and corrosion resistant chemical thick cover physical and chemical plate, they are all the test results is more than American NEMA standards of high quality surface material, is worth you give enough trust, but if you use undeserved, still can cause damage to them, so please pay attention to the matters of attention in the use of the following:

 

1. Don't let the noise of concentration over 95% sulfuric acid or nitric acid and corrosion resistant chemical contact for a long time, especially not to contact for more than 4 hours.

 

2. More than 180 ℃ high temperatures can make the surface layer of corrosion-resistant chemical paper produce color change or foaming phenomenon, so don't let the noise of melting metal, spark and won't know contact with the temperature of the object.

 

3. When using overheating glass or using a coal stove, electric stove, gas stove, or an alcoholic stove, the heat source should be placed on a heat-insulating porcelain or triangular bracket instead of directly touching the table.

 

4. Clean when do not use abrasive, corrosion-resistant chemical containing acid, alkali or corrosion of the cleaner, lest affect surface, usually can use clean water to rinse, for stubborn stains, available hypochlorous acid rinse immediately with water after cleaning, it is strictly prohibited to use sharp metal objects scratch the table. Therefore, good products should be used and maintained in a normal way.

 

Pulsating Vacuum Pressure Steam Sterilizer B-D Test

BD test is used to detect pre-vacuum pressure steam sterilizer cold air discharge test, the test is based on the indicator color change is uniform to determine whether the pulse vacuum sterilizer in the sterilization process has a cold air mass Residual.

 

1 materials and methods

1.1 Materials: 3M company's B-D test paper, made of pure cotton standard package.
1.2 Methods B-D test kit consists of 50 cm × 90 cm cotton cloth towel size of 50, first horizontal towel cloth to 3 layers, and then folded to form a 6 layer. Cloth folded cloth a pile to a height of 25 cm, when stacked, the layers of cloth towels alternately folded around the side so that both sides of the same thickness. Cloth placed, the chemical indicators of the level of folder placed between the central cloth towel, and then cloth all cloth towels into a test bag, the outside with 3M chemical indicator tape fixed, the size of the entire cloth towel bag, about 25 cm high, wide 23 cm, length 27 cm, weight 4 kg.
Place the test pack horizontally in the empty pot and close to the cabinet door and exhaust port. Under the condition of the steam source pressure of 0.30-0.50 MPa and the water source pressure of 0.15-0.30 MPa, the vacuum is evacuated for 3 times with a vacuum of 0.086 MPa Working pressure 0.21 MPa, temperature 134 ℃, sterilization 3.5 ~ 4 min, after removing the judge to determine the results.

 

2 criteria

B-D test paper shows black and white lines clear, uniform color, clear, rapid steam penetration, cold air excluded, no significant air leaks, no cold air mass exists, said the test pass. B-D test paper discoloration uneven color or the middle of the test paper than the external color light, indicating that monitoring failed.

More info, please visit: www.sadalimited.com

Pulse Vacuum Steam Sterilizer Working Principle

Pulse vacuum steam sterilizer referred to as the pre-vacuum pressure cooker. The utility model adopts a vacuum pump to pump the air in the pan into a vacuum state and then introduces the steam so that the steam rapidly diffuses in the pan and penetrates into the deep part of the pan for effective sterilization. It has significant advantages over steam autoclaves:
1. Disinfection time is about 1/3 of the high-pressure steam boiler;
2. Sterilization effect is more reliable than steam pressure sterilizer, and easy to operate;
3. destroy the degree of disinfection light;
4. After sterilization of the dressing package has been close to dry, remove it to use.

More info, please visit: www.sadalimited.com

Pulse Vacuum Autoclave Sterilizer

Portable High Pressure Steam Sterilizer Operation Method

1. Stacking: The drugs to be sterilized are wrapped and then placed sequentially on the sieve plate inside the sterilization bucket with proper clearance between the bag and the package to facilitate the cold air in the sterilization bucket to overflow and steam penetration, improve sterilization.
2. Add water: add 4 liters of clean water in the portable sterilizer body, the water will gradually evaporate in the process of extinguishing, the water level will be reduced accordingly. Therefore, before each sterilization, make up the amount of water inside the sterilizer main body, so as not to dehydration dry electric heater, electric heater damage.
3. Sealing: Put the drum into the pressure steam sterilizer main body, insert the hose on the cover into the semi-circular groove on the inner wall of the sterilizer and close the cover. Aligning the bolt groove on the cover with the bolt groove of the main body, and then sequentially aligning the wing nut in the opposite orientation to the groove to tighten the cover evenly with the main body.
4. Heating: the sterilizer power plug into the 10A / 220V power outlet. Turn the power switch to "on" position, power indicator light, heating tube began to heat. And then the deflation valve plate hand dial to the "deflated" position to be more urgent when the steam is discharged, the deflation valve plate is about to dial to "off" position. At this point pressure gauge pointer clockwise slow rotation, indicating that the pressure inside the sterilizer is gradually rising, while the temperature increases.
5. Sterilization: When the pressure within the sterilizer reaches the desired range, the user can start to calculate the sterilization time, according to different items and packaging, with reference to (Table I) to determine the time required for sterilization. In the sterilization process, the user can sterilize the power switch "on" or "off" to properly adjust the heat source, or connected to a regulator transformer, the appropriate lower voltage so that the pressure inside the sterilizer in the regulator status. However, the pressure inside the sterilizer can not be kept below the user selected value. If the user does not use the above-mentioned method of controlling the heat source, the safety valve will intermittently release the over pressure steam during the sterilization process, and the setting pressure inside the sterilizer is 0.165 MPa at this time.
6. Drying: For medical devices, dressings, utensils and other items that need to be dried quickly after sterilization, the sterilizing steam can be quickly discharged through the bleed valve at the end of sterilization until the pressure gauge hands return to " 0 "scale, and then wait a minute or two after the cover open. Open the cover, and then continue to heat 10 to 15 minutes, so that items on the residual water vapor evaporated. Then turn the power switch to "off" position, stop heating.
7. Cooling: Sterilization of liquid such as medium, etc. After the sterilization is complete, the vaporizer must not be quickly discharged through the vent valve, so as to avoid violent boiling liquid overflow or bottle rupture and other dangerous accidents. Therefore, after sterilizing the sterilized articles of this type, the power switch should be turned to the "OFF" position first, and the heating should be stopped until it is cooled down until the pressure gauge hands return to the "0" scale, and wait a few minutes before releasing Hand valve plate to the "release steam" position. Then you can open the cover.

8. Inspection: Bottom thermometers, sterilization indicators or bacterial culture methods should always be used to check the temperature and time required for sterilization for reliable sterilization.

More info, please visit: www.sadalimited.com.

What Is Pressurized Steam Sterilization

Pressurized steam autoclave sterilizers, sterilization items will be placed in a sealed pressure steam sterilization pot and steam into the pot and the pressure increases, the boiling point of water also will increase Yu. Under normal circumstances, the relationship between temperature and vapor pressure line is as follows:
Different steam pressures attainable temperature
Pressure ( lb / cm2) 5 10 15 20 25 30
Temperature (℃) 108.8 115.6 121.3 127.2 130.4 134.6
In 1.05kg/cm2 (0.1mpa) that the vapor pressure of 15 pounds, the pot temperature reached 121.3 ℃, 30 minutes, including spores were killed, including all bacteria and viruses.

1. Heat sterilization of a factor effect
(1) Temperature: temperature, sterilization time was significantly shortened.
(2) Bacteria: different types and even different strains of bacteria resistant to heat may be different; higher concentration of bacteria, requiring sterilization longer.
(3) medium; heat can affect the nature of the sterilization medium, protein, fat and other bacteria have a protective effect.
(4) pH: bacteria in the neutral medium maximum resistance to heat, easily killed when acidic or slightly alkaline.

2. Should be used with high-pressure steam sterilization precautions
( 1 ) try to exclude the air sterilizers: You must have the device when using autoclaves air drained. If there is the presence of air in the sterilizer, the sterilization temperature will affect reasons: steam mixed with air, it will affect the thermal conduction time of the pressure in the right as shown, and heat is not consistent, and thus resulting in incomplete sterilization. There kiln retained partial retention effect, making it difficult to penetrate the heat, even the formation of temperature stratification, so sterilization should pay close attention to the temperature of the pot. Check that the air drained methods are: install a thermometer in the exhaust port, such as the exhaust gas temperature reaches 100 degrees Celsius when the air has drained pot instructions; hose in the exhaust port set to the other end opening into the cold water basin, and if small bubbles emerging, there is still an air discharge instructions.

( 2 ) controlling the heating Moderate: As a rule, the sterilization time is the time to reach the required sterilization temperature within the piano counting, such as by flash heating, the desired temperature for five minutes, the contents inside the sterilizer 20 minutes after order to achieve the required temperature; as with slow heating, when the 20 minutes to reach the desired temperature sterilization, the sterilization goods in the next five minutes to reach the required temperature. Therefore, the heating temperature sterilization should not be excessive.

( 3 ) do all the work before the prospective sterilization are:
① items prior to sterilization should minimize contamination and maintain the cleanliness of items in order to better ensure its sterilization effect.
② pack ritual items used properly there are both timber farming both good steam penetration, but also to block the outside world only biological invasion, and more generally in brown paper; bandage should not be too tight, to facilitate the flow of steam; installed capacity.
③ sterilized items should not be too full, or about the volume of both 3/ 4, the container hair leaving space for vapor flow.

( 4 ) preferably placed at different locations in the thermometer or temperature indicator sterilization equipment, the sterilization temperature in order to understand the situation. Temperature indicator used to contain benzoic acid and bromine phenol musk orchid (Bromothymol blue) called sterilization certificate. Because formic acid melting point of 121 ℃, such as sterilization temperature reaches 121 ℃, acid melts encounter that bromothymol blue to red. Biological indicators are used to monitor a strong tolerance to heat death of Bacillus stearothermophilus (Bacillus sterothermophilus ATTCC 7953, SSIK 31) death spores to determine the success of sterilization.

More info, please visit: www.sadalimited.com