What Is Pressurized Steam Sterilization

Pressurized steam autoclave sterilizers, sterilization items will be placed in a sealed pressure steam sterilization pot and steam into the pot and the pressure increases, the boiling point of water also will increase Yu. Under normal circumstances, the relationship between temperature and vapor pressure line is as follows:
Different steam pressures attainable temperature
Pressure ( lb / cm2) 5 10 15 20 25 30
Temperature (℃) 108.8 115.6 121.3 127.2 130.4 134.6
In 1.05kg/cm2 (0.1mpa) that the vapor pressure of 15 pounds, the pot temperature reached 121.3 ℃, 30 minutes, including spores were killed, including all bacteria and viruses.

1. Heat sterilization of a factor effect
(1) Temperature: temperature, sterilization time was significantly shortened.
(2) Bacteria: different types and even different strains of bacteria resistant to heat may be different; higher concentration of bacteria, requiring sterilization longer.
(3) medium; heat can affect the nature of the sterilization medium, protein, fat and other bacteria have a protective effect.
(4) pH: bacteria in the neutral medium maximum resistance to heat, easily killed when acidic or slightly alkaline.

2. Should be used with high-pressure steam sterilization precautions
( 1 ) try to exclude the air sterilizers: You must have the device when using autoclaves air drained. If there is the presence of air in the sterilizer, the sterilization temperature will affect reasons: steam mixed with air, it will affect the thermal conduction time of the pressure in the right as shown, and heat is not consistent, and thus resulting in incomplete sterilization. There kiln retained partial retention effect, making it difficult to penetrate the heat, even the formation of temperature stratification, so sterilization should pay close attention to the temperature of the pot. Check that the air drained methods are: install a thermometer in the exhaust port, such as the exhaust gas temperature reaches 100 degrees Celsius when the air has drained pot instructions; hose in the exhaust port set to the other end opening into the cold water basin, and if small bubbles emerging, there is still an air discharge instructions.

( 2 ) controlling the heating Moderate: As a rule, the sterilization time is the time to reach the required sterilization temperature within the piano counting, such as by flash heating, the desired temperature for five minutes, the contents inside the sterilizer 20 minutes after order to achieve the required temperature; as with slow heating, when the 20 minutes to reach the desired temperature sterilization, the sterilization goods in the next five minutes to reach the required temperature. Therefore, the heating temperature sterilization should not be excessive.

( 3 ) do all the work before the prospective sterilization are:
① items prior to sterilization should minimize contamination and maintain the cleanliness of items in order to better ensure its sterilization effect.
② pack ritual items used properly there are both timber farming both good steam penetration, but also to block the outside world only biological invasion, and more generally in brown paper; bandage should not be too tight, to facilitate the flow of steam; installed capacity.
③ sterilized items should not be too full, or about the volume of both 3/ 4, the container hair leaving space for vapor flow.

( 4 ) preferably placed at different locations in the thermometer or temperature indicator sterilization equipment, the sterilization temperature in order to understand the situation. Temperature indicator used to contain benzoic acid and bromine phenol musk orchid (Bromothymol blue) called sterilization certificate. Because formic acid melting point of 121 ℃, such as sterilization temperature reaches 121 ℃, acid melts encounter that bromothymol blue to red. Biological indicators are used to monitor a strong tolerance to heat death of Bacillus stearothermophilus (Bacillus sterothermophilus ATTCC 7953, SSIK 31) death spores to determine the success of sterilization.

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